BST2 impairs Schwann cell function to accelerate age-related hearing loss and serves as a novel serum biomarker

作者: Mengxiao Liu, Huan Cao, Qi Li, Huan Yin, Jiantao Wang, Tao Liu, Lei Zhao, Jianwang Yang, Miaomiao An, Yanan Li, Chen Wang & Baoshan Wang
杂志: Acta Oto-Laryngologica
Doi: https://www.doi.org/10.1080/00016489.2025.2591707
出版日期: 2025/11/30

论文中使用的产品/服务

摘要

Background
Age-related hearing loss (ARHL) represents the most prevalent sensory disorder among the elderly, significantly impairing daily functioning and increasing the risk of psychiatric conditions. Although Schwann cells (SCs) are critical for maintaining auditory nerve function, the mechanisms underlying their dysfunction in ARHL remain incompletely elucidated.

Aims
To investigate BST2’s role in SCs dysfunction in ARHL and assess its potential value as a serum biomarker for early ARHL diagnosis.

Materials and methods
Adeno-associated virus (AAV) carrying a SC specific promoter—MBP, was injected into the posterior semicircular canal of mice. Immunofluorescence was employed to assess cochlear SCs function, while ELISA was used to measured BST2 levels in the serum of both humans and mice.

Results
Overexpression of BST2 inhibited SCs proliferation and differentiation, promoted apoptosis, and accelerated ARHL. Serum BST2 levels were elevated in both ARHL patients and mice, showing a significant positive correlation with audiometric thresholds and wave I latency of ABR.

Conclusions and significance
BST2 accelerates the progression of ARHL by exacerbating SCs dysfunction and serves as a potential serum biomarker for ARHL.

文章链接

关于派真

作为一家专注于AAV 技术十余年，深耕基因治疗领域的CRO&CDMO，派真生物可提供从载体设计、构建到 AAV、慢病毒和 mRNA 服务的一站式解决方案。凭借深厚的技术实力、卓越的运营管理和高标准的服务交付，我们为全球客户提供一站式CMC解决方案，包括从早期概念验证、成药性评估到IIT、IND及BLA的各个阶段。

凭借我们独立知识产权的π-alpha^TM 293 细胞AAV高产技术平台，我们能将AAV产量提高多至10倍，每批次产量可达1×10¹⁷vg，以满足多样化的商业化和临床项目需求。此外，我们定制化的mRNA和脂质纳米颗粒（LNP）产品及服务覆盖药物和疫苗开发的各个阶段，从研发到符合GMP的生产，提供端到端的一站式解决方案。