Cap 1 N1meψ SaCas9HF mRNA
SaCas9HF, or High-Fidelity SaCas9, is a variant of the CRISPR-associated protein Cas9 derived from the bacterium Staphylococcus aureus. Similar to SpCas9HF, SaCas9HF has been engineered to reduce off-target effects and improve specificity when targeting DNA sequences. This variant is designed to enhance accuracy in discriminating between target and non-target DNA sequences, thereby minimizing unintended genetic modifications. SaCas9HF retains the ability to induce double-strand breaks at specific DNA sites, similar to wild-type SaCas9, but with reduced off-target effects. This enhanced fidelity makes SaCas9HF particularly valuable for applications requiring precise genome editing, such as gene therapy and functional genomics research. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.