π-Alpha™ 293 AAV 高产技术平台
![service-banner-950x266](https://www.packgene.com/wp-content/uploads/2020/10/service-banner-950x266-4.jpg)
- 用独特设计的辅助RC质粒,提升293细胞体系AAV的产量可达10倍以上
- 生产工艺极大提升悬浮细胞产量达10~25倍
- 独特工艺极大减少关键杂质(HCD、内毒素等)
- 关键技术降低空壳率、提升感染滴度
Interested in our AAV services? Please contact us for a free consultation or submit a request for a quotation.
![图片1](https://www.packgene.com/wp-content/uploads/2022/05/图片1.png)
![图片2](https://www.packgene.com/wp-content/uploads/2022/05/图片2.png)
提高AAV产量 3~8倍 (通过在RepCap质粒中加入非编码调节元件,已申请专利)
![图片3](https://www.packgene.com/wp-content/uploads/2022/05/图片3.png)
![图片5](https://www.packgene.com/wp-content/uploads/2022/05/图片5.png)
![图片4](https://www.packgene.com/wp-content/uploads/2022/05/图片4.png)
![图片6](https://www.packgene.com/wp-content/uploads/2022/05/图片6.png)
AAV production process using serum free 293 suspension cells (200L)
The pilot process development strategy and parameters for the small batches successfully guide the 200L scale-up AAV production process using serum-free 293 suspension cells.
![图片7](https://www.packgene.com/wp-content/uploads/2022/05/图片7.png)
![图片8](https://www.packgene.com/wp-content/uploads/2022/05/图片8.png)
Actual AAV production up to 7.3E+16 vg (total yield) for 200L production system
T空壳率可低<5%
![图片9](https://www.packgene.com/wp-content/uploads/2022/05/图片9.png)
![图片10](https://www.packgene.com/wp-content/uploads/2022/05/图片10.png)
分析型超高速离心AUC
降低HCD
Reducing the plasmid DNA residue (Packaged plasmid impurity) significantly by unique molecular modification on plasmid
![pranav](https://www.packgene.com/wp-content/uploads/2022/05/pranav.jpg)
![图片11](https://www.packgene.com/wp-content/uploads/2022/05/图片11.png)
![图片12](https://www.packgene.com/wp-content/uploads/2022/05/图片12.png)
![图片13](https://www.packgene.com/wp-content/uploads/2022/05/图片13.png)
Reducing the plasmid DNA residue (Packaged plasmid impurity) significantly by unique molecular modification on plasmid
Scalable process by ultracentrifugation allows 200~500L batch production
Scalable process by ultracentrifugation allows 200~500L batch production
Characters | Descriptions |
High yield | Design of RC plasmid increases AAV yield by 3-8 times |
CPP optimization increases total AAV yields by 10 times | |
Improved scalability | Stable scale-up process: from 125 mL to 200 L (suspension) |
Scalable 200 L suspension process: 7.3E+16 vg yeild, >30% recovery rate | |
Improved biosafety | HCD residue: 30~90 ng/1E13 vg (suspension) |
Optimal full capsid ratio at harvest (20~66%) and at final (75~94%) | |
Novel plasmid design decrease 90% encapsidated plasmid impurity |
π-Alpha™ 293 AAV高产技术平台